Soft sensor for monitoring biomass subpopulations in mammalian cell culture processes
نویسندگان
چکیده
منابع مشابه
Measurement and control of dissolved carbon dioxide in mammalian cell culture processes using an in situ fiber optic chemical sensor.
At high viable cell concentrations in large-scale mammalian cell culture processes, the accumulation of dissolved carbon dioxide (dCO(2), typically quantified as an equilibrium gas-phase concentration) becomes problematic as a result of low CO(2) removal rates at reduced surface-to-volume ratios. High dCO(2) concentrations have previously been shown to inhibit cell growth and product formation ...
متن کاملDecision tree for selection of suitable cultivation parameters for mammalian cell culture processes
Background Development of bioprocesses for mammalian cells has to deal with different bioreactor types and scales. Bio-reactors might be intended for seed train and production, research, process development, validation or transfer purposes. During these activities, not only the problem of upand downscaling might lead to failure of repro-ducibility, but also the use of different bioreactor geome...
متن کاملUniversal Capacitance Model for Real-Time Biomass in Cell Culture
: Capacitance probes have the potential to revolutionize bioprocess control due to their safe and robust use and ability to detect even the smallest capacitors in the form of biological cells. Several techniques have evolved to model biomass statistically, however, there are problems with model transfer between cell lines and process conditions. Errors of transferred models in the declining pha...
متن کاملMammalian Cell Tissue Culture Techniques.
Cultured tissues and cells are used extensively in physiological and pharmacological studies. In vitro cultures provide a means of examining cells and tissues without the complex interactions that would be present if the whole organism were studied. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This unit describe...
متن کاملComparison of spectroscopy technologies for improved monitoring of cell culture processes in miniature bioreactors
Cell culture process development requires the screening of large numbers of cell lines and process conditions. The development of miniature bioreactor systems has increased the throughput of such studies; however, there are limitations with their use. One important constraint is the limited number of offline samples that can be taken compared to those taken for monitoring cultures in large-scal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology Letters
سال: 2017
ISSN: 0141-5492,1573-6776
DOI: 10.1007/s10529-017-2408-0